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Mannosamine, a novel inhibitor of glycosylphosphatidylinositol incorporation into proteins.

1991; Springer Nature; Volume: 10; Issue: 8 Linguagem: Inglês

10.1002/j.1460-2075.1991.tb07726.x

1460-2075

Michael P. Lisanti, Mark C. Field, Ingrid W. Caras, Anant K. Menon, E Rodriguez-Boulan,

Carbohydrate Chemistry and Synthesis

Research Article1 August 1991free access Mannosamine, a novel inhibitor of glycosylphosphatidylinositol incorporation into proteins. M.P. Lisanti M.P. Lisanti Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021. Search for more papers by this author M.C. Field M.C. Field Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021. Search for more papers by this author I.W. Caras I.W. Caras Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021. Search for more papers by this author A.K. Menon A.K. Menon Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021. Search for more papers by this author E. Rodriguez-Boulan E. Rodriguez-Boulan Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021. Search for more papers by this author M.P. Lisanti M.P. Lisanti Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021. Search for more papers by this author M.C. Field M.C. Field Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021. Search for more papers by this author I.W. Caras I.W. Caras Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021. Search for more papers by this author A.K. Menon A.K. Menon Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021. Search for more papers by this author E. Rodriguez-Boulan E. Rodriguez-Boulan Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021. Search for more papers by this author Author Information M.P. Lisanti1, M.C. Field1, I.W. Caras1, A.K. Menon1 and E. Rodriguez-Boulan1 1Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021. The EMBO Journal (1991)10:1969-1977https://doi.org/10.1002/j.1460-2075.1991.tb07726.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Mannosamine (2-amino-2-deoxy D-mannose) is shown here to block the incorporation of glycosylphosphatidylinositol (GPI) into GPI-anchored proteins. The amino sugar drastically reduced the surface expression of a recombinant GPI-anchored protein in polarized MDCK cells, converted this apical membrane-bound protein to an unpolarized secretory product and blocked the expression of endogenous GPI-anchored proteins. Furthermore, it specifically inhibited the incorporation of [3H]ethanolamine (a GPI component) into mammalian and trypanosomal GPI-anchored proteins and into a well characterized GPI-lipid of Trypanosoma brucei. These results suggest that mannosamine converted an apical GPI-anchored protein to a non-polarized secretory product by depleting transfer competent GPI-precursor lipids. Our inhibitor studies provide new independent evidence for the apical targeting role of GPI in polarized epithelia and open the way towards a greater understanding of the functional role of GPI in membrane trafficking and cell regulation. Previous ArticleNext Article Volume 10Issue 81 August 1991In this issue RelatedDetailsLoading ...