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Molecular cloning and expression of the murine interleukin-5 receptor.

1990; Springer Nature; Volume: 9; Issue: 13 Linguagem: Inglês

10.1002/j.1460-2075.1990.tb07886.x

1460-2075

Satoshi Τακακι, A Tominaga, Y Hitoshi, Seiji Mita, Eiichiro Sonoda, Naoto Yamaguchi, Kiyoshi Takatsu,

Cytokine Signaling Pathways and Interactions

Research Article1 December 1990free access Molecular cloning and expression of the murine interleukin-5 receptor. S. Takaki S. Takaki Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author A. Tominaga A. Tominaga Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author Y. Hitoshi Y. Hitoshi Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author S. Mita S. Mita Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author E. Sonoda E. Sonoda Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author N. Yamaguchi N. Yamaguchi Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author K. Takatsu K. Takatsu Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author S. Takaki S. Takaki Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author A. Tominaga A. Tominaga Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author Y. Hitoshi Y. Hitoshi Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author S. Mita S. Mita Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author E. Sonoda E. Sonoda Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author N. Yamaguchi N. Yamaguchi Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author K. Takatsu K. Takatsu Department of Biology, Kumamoto University Medical School, Japan. Search for more papers by this author Author Information S. Takaki1, A. Tominaga1, Y. Hitoshi1, S. Mita1, E. Sonoda1, N. Yamaguchi1 and K. Takatsu1 1Department of Biology, Kumamoto University Medical School, Japan. The EMBO Journal (1990)9:4367-4374https://doi.org/10.1002/j.1460-2075.1990.tb07886.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Murine interleukin-5 (IL-5) is known to play an essential role in Ig production of B cells and proliferation and differentiation of eosinophils. Here, we have isolated cDNA clones encoding a murine IL-5 receptor by expression screening of a library prepared from a murine IL-5 dependent early B cell line. A cDNA library was expressed in COS7 cells and screened by panning with the use of anti-IL-5 receptor monoclonal antibodies. The deduced amino acid sequence analysis demonstrates that the receptor is a glycoprotein of 415 amino acids (Mr 45,284), including an N-terminal hydrophobic region (17 amino acids), a glycosylated extracellular domain (322 amino acids), a single transmembrane segment (22 amino acids) and a cytoplasmic tail (54 amino acids). COS7 cells transfected with the cDNA expressed a 60 kd protein that bound IL-5 with a single class of affinity (KD = 2–10 nM). FDC-P1 cells transfected with the cDNA for murine IL-5 receptor showed the expression of IL-5 binding sites with both low (KD = 6 nM) and high affinity (KD = 30 pM) and acquired responsiveness to IL-5 for proliferation, although parental FDC-P1 cells did not show any detectable IL-5 binding. In addition, several cDNA clones encoding soluble forms of the IL-5 receptor were isolated. Northern blot analysis showed that two species of mRNAs (5.0 kb and 5.8 kb) were detected in cell lines that display binding sites for murine IL-5. Homology search for the amino acid sequence of the IL-5 receptor reveals that the IL-5 receptor contains a common motif of a cytokine receptor family that is recently identified. Previous ArticleNext Article Volume 9Issue 131 December 1990In this issue RelatedDetailsLoading ...