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The Application of LTR Retrotransposons as Molecular Markers in Plants

2012; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-61779-603-6_7

1940-6029

Alan H. Schulman, Andrew J. Flavell, Etienne Paux, Noel Ellis,

Genomics and Phylogenetic Studies

Retrotransposons are a major agent of genome evolution. Various molecular marker systems have been developed that exploit the ubiquitous nature of these genetic elements and their property of stable integration into dispersed chromosomal loci that are polymorphic within species. The key methods, SSAP, IRAP, REMAP, RBIP, and ISBP, all detect the sites at which the retrotransposon DNA, which is conserved between families of elements, is integrated into the genome. Marker systems exploiting these methods can be easily developed and inexpensively deployed in the absence of extensive genome sequence data. They offer access to the dynamic and polymorphic, nongenic portion of the genome and thereby complement methods, such as gene-derived SNPs, that target primarily the genic fraction.