Abstract 15751: The Epigenetic of Becoming a Cardiomyocyte: Elucidating the Link Between HDAC1 and Its Role In Regulating BMP2 Signaling During Cardiovascular Specific Differentiation of Embryonic and Induced Pluripotent Stem Cells
2011; Lippincott Williams & Wilkins; Volume: 124; Linguagem: Inglês
ISSN
1524-4539
AutoresEneda Hoxha, Erin Lambers, Veronica Ramirez, Prasanna Krishnamurthy, Suresh K Verma, Melissa Thal, Raj Kishore,
Tópico(s)CRISPR and Genetic Engineering
ResumoCardiomyocytes derived from embryonic and induced pluripotent stem cells (ES/iPS) provide an excellent source for cell replacement therapies following myocardial ischemia. However, some of the obstacles in the realization of the full potential of iPS/ES cells arise from incomplete and poorly understood molecular mechanisms and epigenetic modifications that govern their cardiovascular specific differentiation. We identified Histone Deacetylase 1 (HDAC1) as a crucial regulator in early differentiation of mES and iPS cells. We propose a novel pathway in which HDAC1 regulates cardiovascular differentiation by regulating BMP2 signaling in differentiating pluripotent cells. Stable HDAC1 knock down mES and iPS clones were created using shRNA vectors. Differentiation through embryoid bodies (EB) was induced in wild type mES cells and iPS cells and in their HDAC1-null counterparts. The ability of these cells to differentiate into cardiovascular lineages was monitored. Compared to wild type cells, HDAC1-null EBs sh...
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