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Topography of intermediates in transcription initiation of E.coli.

1990; Springer Nature; Volume: 9; Issue: 7 Linguagem: Inglês

10.1002/j.1460-2075.1990.tb07391.x

1460-2075

Peter Schickor, Willi Metzger, W. Werel, Hermann Lederer, Hermann Heumann,

RNA and protein synthesis mechanisms

Research Article1 July 1990free access Topography of intermediates in transcription initiation of E.coli. P. Schickor P. Schickor Max Planck Institute of Biochemistry, Martinsried, FRG. Search for more papers by this author W. Metzger W. Metzger Max Planck Institute of Biochemistry, Martinsried, FRG. Search for more papers by this author W. Werel W. Werel Max Planck Institute of Biochemistry, Martinsried, FRG. Search for more papers by this author H. Lederer H. Lederer Max Planck Institute of Biochemistry, Martinsried, FRG. Search for more papers by this author H. Heumann H. Heumann Max Planck Institute of Biochemistry, Martinsried, FRG. Search for more papers by this author P. Schickor P. Schickor Max Planck Institute of Biochemistry, Martinsried, FRG. Search for more papers by this author W. Metzger W. Metzger Max Planck Institute of Biochemistry, Martinsried, FRG. Search for more papers by this author W. Werel W. Werel Max Planck Institute of Biochemistry, Martinsried, FRG. Search for more papers by this author H. Lederer H. Lederer Max Planck Institute of Biochemistry, Martinsried, FRG. Search for more papers by this author H. Heumann H. Heumann Max Planck Institute of Biochemistry, Martinsried, FRG. Search for more papers by this author Author Information P. Schickor1, W. Metzger1, W. Werel1, H. Lederer1 and H. Heumann1 1Max Planck Institute of Biochemistry, Martinsried, FRG. The EMBO Journal (1990)9:2215-2220https://doi.org/10.1002/j.1460-2075.1990.tb07391.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Three characteristic footprinting patterns resulted from probing the Escherichia coli RNA polymerase T7 A1 promoter complex by hydroxyl radicals in the temperature range between 4 degrees C and 37 degrees C. These were attributed to the closed complex, the intermediate complex and the open complex. In the closed complex, the RNA polymerase protects the DNA only at one side over five helical turns. In the intermediate complex, the range of the protected area is extended further downstream by two helical turns. This region of the DNA helix is fully protected, indicating that the RNA polymerase wraps around the DNA between base positions −13 and +20. In the open complex, a stretch between base positions −7 and +2, which was fully protected in the intermediate complex, becomes accessible towards hydroxyl radicals but only in the codogenic strand, indicating that the DNA strands are unwound. Our data suggest that only the DNA downstream of the promoter is involved in this unwinding process. Previous ArticleNext Article Volume 9Issue 71 July 1990In this issue RelatedDetailsLoading ...