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Round-Robin Comparison of Methods for the Detection of Human Enteric Viruses in Lettuce

2004; Elsevier BV; Volume: 67; Issue: 10 Linguagem: Inglês

10.4315/0362-028x-67.10.2315

1944-9097

Françoise S. Le Guyader, Anna Charlotte Schultz, L. Haugarreau, Luciana Croci, Leena Maunula, Erwin Duizer, Froukje Lodder-Verschoor, Carl‐Henrik von Bonsdorff, Elisabetta Suffredini, Wim M.M. Van Der Poel, Rosanna Reymundo, Marion Koopmans,

Animal Virus Infections Studies

Five methods that detect human enteric virus contamination in lettuce were compared. To mimic multiple contaminations as observed after sewage contamination, artificial contamination was with human calicivirus and poliovirus and animal calici-virus strains at different concentrations. Nucleic acid extractions were done at the same time in the same laboratory to reduce assay-to-assay variability. Results showed that the two critical steps are the washing step and removal of inhibitors. The more reliable methods (sensitivity, simplicity, low cost) included an elution/concentration step and a commercial kit. Such development of sensitive methods for viral detection in foods other than shellfish is important to improve food safety.