Portal de Periódicos da CAPES

Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness.

1991; Springer Nature; Volume: 10; Issue: 7 Linguagem: Inglês

10.1002/j.1460-2075.1991.tb07702.x

1460-2075

Bernd Weißhaar, G. A. Armstrong, A. Block, O. da Costa e Silva, Klaus Hahlbrock,

Plant Molecular Biology Research

Research Article1 July 1991free access Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness. B. Weisshaar B. Weisshaar Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author G.A. Armstrong G.A. Armstrong Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author A. Block A. Block Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author O. da Costa e Silva O. da Costa e Silva Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author K. Hahlbrock K. Hahlbrock Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author B. Weisshaar B. Weisshaar Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author G.A. Armstrong G.A. Armstrong Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author A. Block A. Block Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author O. da Costa e Silva O. da Costa e Silva Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author K. Hahlbrock K. Hahlbrock Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author Author Information B. Weisshaar1, G.A. Armstrong1, A. Block1, O. Costa e Silva1 and K. Hahlbrock1 1Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. The EMBO Journal (1991)10:1777-1786https://doi.org/10.1002/j.1460-2075.1991.tb07702.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Four cis-acting elements, designated as Boxes I, II, III and IV, have previously been identified as functionally relevant components of the light-responsive chalcone synthase (CHS) promoter in parsley (Petroselinum crispum). This paper describes the isolation of three cDNAs encoding proteins which bind specifically to Box II, one of two cis-acting elements found within a 52 bp CHS promoter region shown here to be sufficient for light responsiveness in parsley. The deduced amino acid sequences of all three proteins reveal conserved basic and leucine zipper domains characteristic of transcription factors of the bZIP class. Nucleotide sequences recognized by these factors contain an ACGT motif common to many cis-acting elements. Therefore, we have termed the proteins CPRF-1, −2 and −3 (Common Plant Regulatory Factor). The characteristics of CPRF-1 binding to Box II and the timing of transient CPRF-1 mRNA accumulation during light exposure of previously dark-grown parsley cells are consistent with the hypothesis that this factor participates in the light-mediated activation of the CHS gene in parsley. Previous ArticleNext Article Volume 10Issue 71 July 1991In this issue RelatedDetailsLoading ...