Portal de Periódicos da CAPES

Fatty acid profile and cholesterol content in muscle tissue of one year old common carp (Cyprinus carpio) during growth.

2009; Volume: 50; Linguagem: Inglês

2406-1247

Dejana Trbović, Danijela Vranić, Jasna Đinović, Branka Borović, Danka Spirić, Jelena Babić, Aurelija Spirić,

Fish Biology and Ecology Studies

Despite the fact that possibilities for fishery development in Serbia are favorable considering the bioecological properties of the region, fish production is insufficient. Predominant form of national aquaculture is semi intensive breeding system of cyprinidae, making over 80% of total fish production. However, increasing demand and consumption of aqua cultured fish on national and international level is accompanied with the certain demands concerning quality and nutritive properties, i.e. content of polyunsaturated fatty acids. Having in mind these facts, the aim of this paper was to investigate chemical and fatty acids composition of both fish filets and feed, as well as cholesterol content of common carp (Cyprinus carpio) in the final breeding stage during spring and summer (April and June). Studying these parameters in the production stage will allow the insight in all significant factors in order to obtain high quality aqua cultured products. Results obtained for the chemical parameters of feed (protein, moisture, fat and ash content) lead to the conclusion that the chemical composition was approximately the same in April and June. Chemical composition of carp filets is also steady during this period. It is also determined that cholesterol content in carp filets is significantly different in April and June (48,87 mg/100g in April and 54,31 mg/100g in June). Statistical analysis was carried out by applying Student's t-test and comparing the average values of cholesterol content during the experiment. Fatty acids content in feed samples has been correlated with the fatty acids content of the carp filets and the calculated values of Parson's correlational coefficient for April and June were : r = 0,8187 and r = 0,8026 respectively. The corresponding values of t parameter are 5,14 i 4,85 (tcrit = 2,16, P = 0,05), which points out to the significant correlation between fatty acids composition of feed and investigated fish samples. In order to establish the differences between 13 samples of fish (6 and 7 samples from each period), it was necessary to carry out the principal component analysis (PCA). The results clearly suggest that the existence of two groups of data, separated by the sampling time (April and June). To confirm the differences between these two groups, Hotteling's T2 test was conducted in order to compare two populations of multivariate objects (fish samples). Taken variables were the score values for the first six principal components. The value of T2 was 136,911, p < 0,001 which clearly indicates the differences between these two groups of samples, i.e. differences in fatty acids composition of fish muscle samples taken in April and June. On the basis of fatty acids composition of carp filets, the content of n-3 and n-6 polyunsaturated fatty acids was determined as well as n-3/n-6 ratio in carp filets which, in April, was 0,19 (n-3:n-6=1:5,3), and for June 0,28 (n-3:n-6=1:3,6). Ratio of n-3/n-6 in feed was 0,06 (n-3:n-6=1:15,8) in April, and 0,05 (n-3:n-6=1:18,9) in June. The content of n-3 and n-6 PUFA per 100g of carp filets was calculated, as well as the content of EPA and DHA, and their respective values are: 115,42 mg/100g; 562,05 mg/100g; 18,90 mg/100g and 24,07 mg/100g in April and 195,45 mg/100g; 685,75 mg/100g; 22,75 mg/100g and 19,20 mg/100g in June. .