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Permeability problems encountered when treating conidia of with RNA synthesis inhibitors

1972; Elsevier BV; Volume: 48; Issue: 4 Linguagem: Inglês

10.1016/0006-291x(72)90680-8

1090-2104

J. H. Tisdale, A. G. DeBusk,

Botanical Research and Chemistry

Conidia of Neurospora crassa were treated with three inhibitors of RNA synthesis. These cells are impermeable to actinomycin D and alpha-amanitin. To overcome this problem, the cells were treated with EDTA. It was found that EDTA prevents the uptake of radioactive RNA precursors, resulting in an apparent inhibition of RNA synthesis. Evidence is also presented that the inhibitor, proflavine, may alter permeability of conidia.