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BIBTEX RIS

Laticifer proteins from Plumeria pudica inhibit the inflammatory and nociceptive responses by decreasing the action of inflammatory mediators and pro-inflammatory cytokines

2015; Springer Science+Business Media; Volume: 25; Issue: 3 Linguagem: Inglês

10.1016/j.bjp.2015.05.003

ISSN

1981-528X

Autores

Heliana B. Fernandes, Douglas L. Machado, Jordana Maia Dias, Tarcísio Vieira de Brito, Jalles Arruda Batista, Renan O. Silva, Anna Carolina Toledo da Cunha Pereira, Gustavo Portela Ferreira, Márcio V. Ramos, Jand Venes Rolim Medeiros, Karoline S. Aragão, Ronaldo A. Ribeiro, André Luiz dos Reis Barbosa, Jefferson Soares de Oliveira,

Tópico(s)

Cynara cardunculus studies

Resumo

Some publications have described the pharmacological properties of latices proteins.Thus, in the present study proteins from Plumeria pudica Jacq., Apocynaceae, latex were evaluated for anti-inflammatory and antinociceptive activities.Obtained data showed that an intraperitoneal administration of different doses of latex was able to reduce the paw edema induced by carrageenan in a dose-dependent manner (better dose 40 mg/kg; 72.7% inhibition at 3rd and 78.7% at 4 th hour) and the edema induced by dextran (40 mg/kg; 51.5% inhibition at 30 min and 93.0% at 1 st hour).Inhibition of edema induced by carrageenan was accompanied by a reduction of myeloperoxidase activity.Pre-treating animals with latex (40 mg/kg) also inhibited the paw edema induced by histamine, serotonin, bradykinin, prostaglandin E 2 , compound 48/80.Additionally, the latex (40 mg/kg) reduced the leukocyte peritoneal migration induced by carrageenan and this event was followed by reduction of IL-1␤ and TNF-␣ in peritoneal fluid.The latex-treatment (40 mg/kg) reduced the animal abdominal constrictions induced by acetic acid and the first phase on paw licking model induced by formalin.When latex was treated with heat (at 100 • C for 30 min), anti-edematogenic and myeloperoxidase activities were significantly reduced, indicating the involvement of heat-sensitive proteins on anti-inflammatory effect.Our results evidence that latex fluids are a source of proteins with pharmacological properties.

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