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First isolation of a new species of Leishmania responsible for human cutaneous leishmaniasis in Ghana and classification in the Leishmania enriettii complex

2015; Elsevier BV; Volume: 45; Issue: 11 Linguagem: Inglês

10.1016/j.ijpara.2015.05.001

1879-0135

Godwin Kwakye-Nuako, Mba-Tihssommah Mosore, Christopher Duplessis, Michelle Bates, Naiki Puplampu, Israel Mensah-Attipoe, Kwame Desewu, Godwin Afegbe, Richard Harry Asmah, Manal B. Jamjoom, Patrick F. Ayeh‐Kumi, Daniel A. Boakye, Paul A. Bates,

Research on Leishmaniasis Studies

An active case detection approach with PCR diagnosis was used in the Ho District of the Volta Region, Ghana that identified individuals with active cutaneous leishmaniasis. Three isolates were successfully cultured and DNA sequences from these were analysed (ribosomal RNA internal transcribed spacer 1; ribosomal protein L23a intergenic spacer; RNA polymerase II large subunit), showing them to be Leishmania, identical to each other but different from all other known Leishmania spp. Phylogenetic analysis showed the parasites to be new members of the Leishmania enriettii complex, which is emerging as a possible new subgenus of Leishmania parasites containing human pathogens.