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Pyrenophora teres genetic diversity as detected by ISSR analysis.

2010; Lithuanian Research Centre for Agriculture and Forestry; Volume: 97; Issue: 4 Linguagem: Inglês

2335-8947

G. Statkevičiu ̄tė, Gintaras Brazauskas, Roma Semaškienė, Algė Leistrumaitė, Zenonas Dabkevičius,

Yeasts and Rust Fungi Studies

Specific PCR and inter simple sequence repeat (ISSR) marker systems were used to study genetic diversity in barley pathogen Pyrenophora teres isolates collected in 5 districts of Lithuania during 2006–2009. Specific PCR was used to determine the net/spot form and mating type occurrence in net blotch field populations. The occurrence of mating types and net/spot were investigated in all years/ locations, however only Pyrenophora teres f. teres was detected in all investigated populations. Both mating types were found and their ratio was 1:1 in all populations except for Klaipėda distr. 2008. Both mating types were found in Klaipėda population as well, but the observed ratio did not meet the expected. A total of 24 isolates from Kėdainiai and Marijampolė distr. 2007–2008 were selected for ISSR analysis. Primers UBC807 and G11 were most suitable. The selected primers amplified 31 bands in total within the range of 500 bp to 2500 bp and all isolates had unique ISSR haplotypes. Analysis of molecular variance (AMOVA) detected most of the variation within populations – 88.78%, while the variation between populations from the same location in different years accounted for 6.37%, and the variation between different locations in the same year was the smallest – 4.85% of the total variation. No clear grouping according to location or year of sampling was detected in cluster analysis.