Silver Staining of Gels for Proteins or RNA
1986; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-444-01082-7.50084-9
AutoresLeonard G. Davis, Mark D. Dibner, James F. Battey,
Tópico(s)RNA and protein synthesis mechanisms
ResumoThis chapter describes a sensitive and efficient way to visualize the ribonucleic acid (RNA) or protein bands on polyacrylamide gels. Two methods are presented for this purpose: the use of a kit and a less expensive laboratory method. For RNA, the polyacrylamide gel is run on an electrophoresis apparatus. For protein, the gel is run on a 1.0–1.5 mm gel. In the kit method, after running electrophoresis, the gel is placed in plastic dish with 400 ml of 40% methanol. Reagents are changed in the dish at the indicated times, volumes, and solutions. Bands develop during the last three steps of the process. The time of band development can vary. The stop solution is added before the background becomes darker than the desired bands. After stopping, the gel is washed in 400 ml of water. The gel can be photographed or used for other analysis and stored in a sealed plastic bag.
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