A new assay for protoporphyrinogen oxidase — Evidence for a total deficiency in that activity in a heme-less mutant of Saccharomyces cerevisiae
1982; Elsevier BV; Volume: 106; Issue: 3 Linguagem: Inglês
10.1016/0006-291x(82)91771-5
ISSN1090-2104
AutoresJean‐Michel Camadro, Danièle Urban‐Grimal, Pierre Labbé,
Tópico(s)Hemoglobin structure and function
ResumoA new spectrophotometric assay for protoporphyrinogen oxidase activity has been developed, involving enzymatic generation of protoporphyrinogen in the incubation medium. This assay, more sensitive and reliable than those previously described, can be used to measure this activity in yeast mitochondrial membranes, rat liver mitochondria and E. coli membranes. By measuring protoporphyrinogen oxidase activity in different wild type and heme-mutant yeast strains, it was shown that 1) one heme-mutant was totally lacking this activity, 2) different factors might control its level in yeast.
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