Avaliação do potencial antimutagênico de extrato etanólico de própolis verde e de Baccharis dracunculifolia (Asteraceae), por meio de sistema-teste de Allium e células de mamíferos (HTC)
2009; Linguagem: Inglês
ISSN
2639-6459
Autores Tópico(s)Pharmaceutical and Antibiotic Environmental Impacts
ResumoConsumption of apicultural products has attracted the interest of the population due to the practice of natural medicine. Bees collect substances and fragments of vegetal origin to produce propolis, which is used to seal and protect the beehive against pathogenic microorganisms. Human beings use the propolis since the antiquity and for several purposes. By extensive studies, nowadays it is known that there are specific types of propolis, which vary between geographic regions and botanical sources and, therefore, present specific chemical compositions. The Brazilian green propolis, which has recently received international prominence, has as botanical source the plant Braccharis dracunculifolia (Asteraceae), popularly known as alecrim-do-campo. Currently, ethanolic extracts of natural products, eg made with green propolis, have been highlighted for their therapeutical properties. In the present work, the cytotoxic, genotoxic and mutagenic potentials were evaluated, but, mainly, it was verified the possible anticytotoxic, antigenotoxic and antimutagenic activities of these ethanolic extracts in the Allium cepa (onion) test system and in HTC (hepatoma tissue culture) cells maintained in culture. The verification of the mitotic index, of meristematics cells of A. cepa, did not serve as parameter to evaluate cytotoxicity and anticytotoxicity. The analyses of chromosomal aberrations (AC) and micronuclei (MN) frequencies revealed that, both extracts, were not genotoxic nor mutagenic for meristematics cells of A. cepa. Also, there was no significant induction of MN in onion F1 cells and HTC cells. The comet assay, carried out with HTC cells, indicated absence of genotoxic action for the extracts tested in this work. The pre-treatment carried out with cells of A. cepa showed that the extracts were capable to reduce the induction of cytogenetics damages promoted by Methyl Methane Sulfonate (MMS), for meristematic cells and F1 cells. The simultaneous treatment, performed with pre-incubated samples and applied in HTC cells, demonstrated significant reduction in the MN frequency. By the obtained results, we can infer that ethanolic extracts of green propolis and Baccharis dracunculifolia are capable to reduce the induction of damages, promoted by genotoxic and/or mutagenic agents, in the genetic material of Allium cepa and HTC cells. LISTA DE ABREVIACOES AC Aberracoes Cromossomicas CCD Cromatografia em camada delgada CHO Chinese Hamster Ovary cells ou celulas de ovario de hamster chines CN Controle Negativo CP Controle Positivo CS Controle do Solvente DMSO Dimetilsulfoxido DP Desvio padrao EDTA Ethylenediaminetetraacetic acid ou acido etilenodiamino tetra-acetico EEBD Extrato etanolico de Baccharis dracunculifolia EEPV Extrato etanolico de propolis verde HMB Human melanoma Black cells ou celulas de melanoma humano HTC Hepatoma Tissue Culture ou cultura celular de hepatoma IM Indice Mitotico MMS Metilmetano Sulfonato MN Micronucleo PBS Phosphate Buffered Saline ou tampao fosfato Rf Fator de retencao RNS Reactive Nitrogen Species ou Especies reativas de nitrogenio ROS Reactive Oxygen Species ou Especies reativas de oxigenio SAR Structure-activity Relationships ou relacao estrutura-atividade SOD Superoxido desmutase TMNBC Teste do micronucleo com bloqueio de citocinese
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