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Bioconjugation of gold-polymer core–shell nanoparticles with bovine serum amine oxidase for biomedical applications

2015; Elsevier BV; Volume: 134; Linguagem: Inglês

10.1016/j.colsurfb.2015.06.052

1873-4367

Iole Venditti, Taha F. Hassanein, Ilaria Fratoddi, Lucia Fontana, Chiara Battocchio, Federica Rinaldi, Maria Carafa, Carlotta Marianecci, Marco Diociaiuti, Enzo Agostinelli, C. Cametti, Mario Vincenzo Russo,

Hemoglobin structure and function

Core–shell gold nanoparticles [AuNPs], stabilized with a hydrophilic polymer, poly(3-dimethylammonium-1-propyne hydrochloride) [PDMPAHCl], have been used for the immobilization of bovine serum amine oxidase [BSAO]. The functionalized surface of the hybrid nanoparticles is pH responsive, due to the presence of aminic groups that carry out a double role: on one hand they act as ligands for the gold nanoparticle surface, allowing the colloidal stabilization and, on the other hand, they give a hydrophilic characteristic to the whole colloidal suspension. The core–shell nanoparticles [[email protected]] have been characterized by using UV–vis and X-ray photoelectron spectroscopy, DLS, ζ-potential measurements and by FE-TEM microscopy. BSAO enzyme can be loaded by non-covalent immobilization onto [email protected] nanoparticles up to 70% in weight, depending on the pH values of the environmental medium. Activity tests on [email protected] bioconjugates confirm an enzymatic activity up to 40%, with respect to the free enzyme activity. Moreover, our results show that loading and enzymatic activity are rather interrelated characteristics and that, under appropriate polymer concentration and pH conditions, a satisfactory compromise can be reached. These results, as a whole, indicate that [email protected] bioconjugate systems are promising for future biomedical applications.