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RNA-seq analysis evidences multiple gene responses in Caligus rogercresseyi exposed to the anti-salmon lice drug azamethiphos

2015; Elsevier BV; Volume: 446; Linguagem: Inglês

10.1016/j.aquaculture.2015.05.011

1873-5622

Valentina Valenzuela-Muñóz, Jacqueline Chávez-Mardones, Cristian Gallardo‐Escárate,

Insect Resistance and Genetics

Azamethiphos is an organophosphate compound used in controlling Caligus rogercresseyi infestations in the Chilean salmon industry. The molecular target of organophosphates has traditionally been associated with acetylcholinesterase (AChE) activity in the nervous system. However, in arthropods these chemicals could induce complex genomic responses, which can involve different molecular pathways not exclusively related to the nervous system. Our study presents a transcriptomic analysis of C. rogercresseyi exposed to azamethiphos and identifies novel candidate genes that were highly deregulated as a result of this delousing drug. Moreover, Illumina sequencing was performed for adult male and female salmon lice exposed to 3 ppb of azamethiphos. RNA-seq analysis evidenced high up-regulation for genes such as the cuticle protein, metalloproteinase, trypsin, myosin, NADH dehydrogenase, ABC transporters, and glutathione S-transferase. Furthermore, SNP mining was carried out in genes with different transcription levels among treatments and sexes. The present study provides relevant genomic information for C. rogercresseyi in response to azamethiphos, thus allowing for the identification of genes putatively associated with the resistance/susceptibility to organophosphates in this species. The manuscript describes a transcriptome expression of Caligus rogercresseyi exposed to the delousing drug Azamethiphos. The study gathers high-throughput sequencing features to suggest novel aspects of the drug response to organophosphates used by the salmon aquaculture industry.