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RNA interference in adult Ascaris suum – an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite

2015; Elsevier BV; Volume: 45; Issue: 11 Linguagem: Inglês

10.1016/j.ijpara.2015.05.003

1879-0135

Ciaran J. McCoy, Neil D. Warnock, Louise E. Atkinson, Erwan Atcheson, Richard J. Martin, Alan P. Robertson, Aaron G. Maule, Nikki J. Marks, Angela Mousley,

Helminth infection and control

The sustainable control of animal parasitic nematodes requires the development of efficient functional genomics platforms to facilitate target validation and enhance anthelmintic discovery. Unfortunately, the utility of RNA interference (RNAi) for the validation of novel drug targets in nematode parasites remains problematic. Ascaris suum is an important veterinary parasite and a zoonotic pathogen. Here we show that adult A. suum is RNAi competent, and highlight the induction, spread and consistency of RNAi across multiple tissue types. This platform provides a new opportunity to undertake whole organism-, tissue- and cell-level gene function studies to enhance target validation processes for nematode parasites of veterinary/medical significance.