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Detection of Apoptosis by TUNEL Assay

2012; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-61779-860-3_5

1940-6029

Kateryna Kyrylkova, Sergiy Kyryachenko, Mark Leid, Chrissa Kioussi,

Advanced biosensing and bioanalysis techniques

Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.