Characterization and biological activities of a novel polysaccharide isolated from raspberry ( Rubus idaeus L.) fruits
2015; Elsevier BV; Volume: 132; Linguagem: Inglês
10.1016/j.carbpol.2015.06.068
ISSN1879-1344
AutoresZeyuan Yu, Lu Liu, Yaqin Xu, Libo Wang, Xin Teng, Li X, Jing Dai,
Tópico(s)Advanced Glycation End Products research
ResumoA water-soluble polysaccharide namely RCP-II from raspberry fruits was obtained by complex enzyme method followed by successive purification using macroporous resin D4020 and Sephadex G-100 columns. RCP-II was an acidic heteropolysaccharide and the characteristic structure of polysaccharide was determined. The carbohydrate of RCP-II was composed with galacturonic acid, rhamnose, arabinose, xylose, glucose and galactose in a molar ratio of 1.00:0.55:1.19:0.52:0.44:1.90 and the average molecular weight was estimated to be 4013 Da, based on dextran standards. RCP-II presented high scavenging activity toward DPPH•, HO•, O2•− in a concentration-dependent manner. The determination of the inhibitory activity on protein glycation showed that in 14 days of incubation the inhibitory ability of RCP-II was more effective on the development of non-enzymatic glycation reaction at early phase than that at the following two phases.
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