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T cell development in mice lacking the CD3-zeta/eta gene.

1993; Springer Nature; Volume: 12; Issue: 11 Linguagem: Inglês

10.1002/j.1460-2075.1993.tb06119.x

1460-2075

Marie Malissen, A. Gillet, Bénédita Rocha, Jeannine Trucy, Éric Vivier, Catherine Boyer, Frank Köntgen, Nicole Brun, Gilbert Mazza, Eugenia Spanopoulou,

Immunotherapy and Immune Responses

Research Article1 November 1993free access T cell development in mice lacking the CD3-zeta/eta gene. M. Malissen M. Malissen Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author A. Gillet A. Gillet Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author B. Rocha B. Rocha Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author J. Trucy J. Trucy Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author E. Vivier E. Vivier Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author C. Boyer C. Boyer Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author F. Köntgen F. Köntgen Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author N. Brun N. Brun Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author G. Mazza G. Mazza Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author E. Spanopoulou E. Spanopoulou Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author M. Malissen M. Malissen Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author A. Gillet A. Gillet Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author B. Rocha B. Rocha Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author J. Trucy J. Trucy Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author E. Vivier E. Vivier Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author C. Boyer C. Boyer Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author F. Köntgen F. Köntgen Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author N. Brun N. Brun Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author G. Mazza G. Mazza Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author E. Spanopoulou E. Spanopoulou Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. Search for more papers by this author Author Information M. Malissen1, A. Gillet1, B. Rocha1, J. Trucy1, E. Vivier1, C. Boyer1, F. Köntgen1, N. Brun1, G. Mazza1 and E. Spanopoulou1 1Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France. The EMBO Journal (1993)12:4347-4355https://doi.org/10.1002/j.1460-2075.1993.tb06119.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The CD3-zeta and CD3-eta polypeptides are two of the components of the T cell antigen receptor (TCR) which contribute to its efficient cell surface expression and account for part of its transducing capability. CD3-zeta and CD3-eta result from the alternative splicing of a single gene designated CD3-zeta/eta. To evaluate the role of these subunits during T cell development, we have produced mice with a disrupted CD3-zeta/eta gene. The analysis of thymocyte populations from the CD3-zeta/eta-/− homozygous mutant mice revealed that they have a profound reduction in the surface levels of TCR complexes and that the products of the CD3-zeta/eta gene appear to be needed for the efficient generation and/or survival of CD4+CD8+ thymocytes. Despite the almost total absence of mature single positive thymocytes, the lymph nodes from zeta/eta-/− mice were found to contain unusual CD4+CD8- and CD4-CD8+ single positive cells which were CD3-. In contrast to the situation observed in the thymus, the thymus-independent gut intraepithelial lymphocytes present in zeta/eta-/− mice do express TCR complexes on their surface and these are associated with Fc epsilon RI gamma homodimers. These results establish an essential role for the CD3-zeta/eta gene products during intrathymic T cell differentiation and further emphasize the difference between conventional T cells and thymus-independent gut intraepithelial lymphocytes. Previous ArticleNext Article Volume 12Issue 111 November 1993In this issue RelatedDetailsLoading ...