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Fatal systemic toxoplasmosis in Valley quail (Callipepla californica)

2015; Elsevier BV; Volume: 4; Issue: 2 Linguagem: Inglês

10.1016/j.ijppaw.2015.04.003

2213-2244

Renata Assis Casagrande, Hilda Fátima Jesus Pena, Aline Diniz Cabral, Verônica Machado Rolim, Luiz Gustavo Schneider de Oliveira, Fabiana M. Boabaid, Angélica Terezinha Barth Wouters, Flademir Wouters, Cláudio Estêvão Farias Cruz, David Driemeier,

Mosquito-borne diseases and control

An adult, captive raised male Valley quail (Callipepla californica) acquired by a southern Brazilian aviary suddenly showed severe apathy, dyspnea and diarrhea, and died 18 hours after the onset of illness. At necropsy, pale muscles and whitish areas in the heart, splenomegaly, hepatomegaly, and consolidated red lungs were observed. Histological findings were mainly mononuclear inflammation with necrosis of liver, heart, spleen, bone marrow and lung. There were large numbers of Toxoplasma gondii tachyzoitesorganisms in the liver, heart, spleen, bone marrow, lungs, trachea, kidneys, adrenal glands, testes, intestines, and pancreas. These organisms were seen free in the organs' stroma or within macrophages and stained positively with polyclonal antiserum to T. gondii. Genomic DNA was extracted from the tissues and PCR was used to target the B1 gene of T. gondii. The genotypic characterization by PCR-RFLP with 11 markers (SAG1, SAG2 and alt. SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3) revealed the ToxoDB-PCR-RFLP #87 genotype, the same as previously identified in a backyard chicken (TgCkBr156) in Rio Grande do Sul, Brazil.