Portal de Periódicos da CAPES

Isolation of Proteins Associated with the DNA-Bound Estrogen Receptor α

2009; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-60327-378-7_13

1940-6029

Jennifer R. Schultz-Norton, Yvonne Ziegler, Varsha S. Likhite, Ann M. Nardulli,

Cancer therapeutics and mechanisms

Regulating gene expression is a complex process requiring the interaction of multiple transcription factors with their cognate recognition sequences. While these DNA-bound transcription factors are the primary drivers of gene expression, the capacity of a transcription factor to alter gene expression is tempered by its association with a host of coregulatory proteins that are recruited to the DNA-bound transcription factor. We have developed a novel approach to isolate large complexes of proteins associated with the DNA-bound estrogen receptor α (ERα) using an agarose-based electrophoretic mobility shift assay (EMSA). This method should be readily adapted to a variety of cultured cell lines, DNA sequences, and transcription factors and has the potential to provide valuable information about a wide variety of regulatory proteins involved in influencing gene expression.