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Quantitative Histological Studies on Aging Changes in Cerebral Cortex of Rhesus Monkey and Albino Rat with Notes on Effects of Prolonged Low-Dose Ionizing Irradiation in the Rat

1973; Elsevier BV; Linguagem: Inglês

10.1016/s0079-6123(08)60684-6

1875-7855

Kenneth R. Brizzee,

Effects of Radiation Exposure

This chapter focuses on the quantitative histological studies on aging changes in cerebral cortex of rhesus monkey and albino rat with notes on effects of prolonged low-dose ionizing irradiation in the rat. The chapter compares three methods of cell enumeration, first the method based on the amount of DNA-P in the cortical tissue, second the traditional technique of counting cells in fixed-stained tissues with the use of a grid reticule (counting chamber method), and thirdly a cell maceration method adapted from the technique of Nurnberger and Gordon. Results obtained from cell counts in fixed-stained tissues (with corrections for tissue shrinkage during processing) and from the DNA-P method in the rat somatosensory cerebral cortex were in good agreement in regard to total cell counts per unit volume of fresh tissue. In electron micrographs, an increase in the amount of glycogen granules in astrocyte cell processes in cerebral cortex of irradiated middle-age rats was noted, but there was no evidence of any other ultrastructural alterations in cerebral cortex of rats or monkeys.