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Nanomaterial-assisted PCR based on thermal generation from magnetic nanoparticles under high-frequency AC magnetic fields

2015; Elsevier BV; Volume: 635; Linguagem: Inglês

10.1016/j.cplett.2015.06.070

1873-4448

Toshiaki Higashi, Hiroaki Minegishi, Akinobu Echigo, Yutaka Nagaoka, Takahiro Fukuda, Ron Usami, Toru Maekawa, Tatsuro Hanajiri,

Innovative Microfluidic and Catalytic Techniques Innovation

Here the authors present a nanomaterial-assisted PCR technique based on the use of thermal generation from magnetic nanoparticles (MNPs) under AC magnetic fields. In this approach, MNPs work as internal nano thermal generators to realize PCR thermal cycling. In order to suppress the non-specific absorption of DNA synthetic enzymes, MNPs are decorated with bovine serum albumin (BSA), forming BSA/MNP complexes. Under high-frequency AC magnetic fields, these complexes work as internal nano thermal generators, thereby producing the typical temperature required for PCR thermal cycling, and perform all the reaction processes of PCR amplification in the place of conventional PCR thermal cyclers.