Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Gelatin and galactomannan-based scaffolds: Characterization and potential for tissue engineering applications

2015; Elsevier BV; Volume: 133; Linguagem: Inglês

10.1016/j.carbpol.2015.06.039

1879-1344

Nataly Machado Siqueira, Bruno Paiva dos Santos, Melissa Camassola, Emily Q. Rosenthal‐Kim, Ketlin C. Garcia, Francisco P. dos Santos, Rosane Michele Duarte Soares,

Collagen: Extraction and Characterization

In this work, we produced gelatin films containing different concentrations of galactomannan by casting solutions. The films were crosslinked by immersion in 30mM solution of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC). The crosslinking of gelatin-containing films was confirmed by the reduction of free amine band intensity (3400-3200cm(-1)) in the GEL IR, as well as by the evaluation of its behavior when immersed in phosphate-buffer solution. The crosslinking of galactomannan film was confirmed by the formation of new ether bonds, as observed by increasing intensity of the band at 1148cm(-1), and the reduction of OH band intensity (3600-3200cm(-1)). The presence of galactomannan and the crosslinking mediated by EDC were responsible to improve elasticity in the gelatin-based films. The samples did not show cytotoxicity during 24h or 48h. In addition, rat mesenchymal stem cells adhered to the films regardless of galactomannan concentration. The results indicated that the gelatin/galactomannan films are potential biomaterials for use as scaffolds for tissue engineering.