Comparison of the brine shrimp nauplii bioassay and the ToxScreen-II Test for the detection of toxicity associated with Aloe vera ( Aloe barbadensis Miller) leaf extract
2009; Medknow; Volume: 1; Issue: 2 Linguagem: Inglês
ISSN
0976-4836
AutoresIan Edwin Cock, D. R. Ruebhart,
Tópico(s)Pharmaceutical and Antibiotic Environmental Impacts
ResumoThis study assessed the performance of a bioluminescent bacterial assay, the ToxScreen-II Test ( Photobacterium leiognathi ), in comparison to the brine shrimp ( Artemia franciscana ) nauplii lethality assay for the detection of toxicity associated with Aloe vera leaf gel extract and the organophosphate pesticide Mevinphos. In both bioassays, clear dose-response relationships were demonstrated for these two test substances. Sensitivity to the Aloe vera leaf extract was similar for both bioassays, with 1 h IC 50 of 2 994 )g/ml in the the ToxScreen-II Test and 2 h LC 50 of 3 940 )g/ml in the brine shrimp nauplii bioassay. In contrast, the ToxScreen-II Test was approximately 10 fold more sensitivity towards Mevinphos (1 h IC 50 113 )g/ml) than the brine shrimp nauplii test (24 h LC 50 1 316). While the ToxScreen-II Test was developed as a tool primarily for the detection of anthropogenic chemicals in water, this study showed that it can also be used for the screening of bioactivity in a complex plant extract. The advantages of this bioluminescent bacterial assay, over the traditionally used brine shrimp test, included a quicker turn around time, reduced preparation of the test organism and other materials, and the acquisition of test results within 1 hour.
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