Portal de Periódicos da CAPES

A mutant cysteinyl-tRNA synthetase affecting timing of chromosomal replication initiation in B. subtilis and conferring resistance to a protein kinase C inhibitor.

1994; Springer Nature; Volume: 13; Issue: 10 Linguagem: Inglês

10.1002/j.1460-2075.1994.tb06532.x

1460-2075

Simone J. Séror, Serge Casarégola, F. Vannier, Naïla Zouari, Michael K. Dahl, Erik Boye,

RNA and protein synthesis mechanisms

Research Article15 May 1994free access A mutant cysteinyl-tRNA synthetase affecting timing of chromosomal replication initiation in B. subtilis and conferring resistance to a protein kinase C inhibitor. S.J. Séror S.J. Séror Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author S. Casarégola S. Casarégola Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author F. Vannier F. Vannier Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author N. Zouari N. Zouari Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author M. Dahl M. Dahl Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author E. Boye E. Boye Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author S.J. Séror S.J. Séror Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author S. Casarégola S. Casarégola Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author F. Vannier F. Vannier Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author N. Zouari N. Zouari Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author M. Dahl M. Dahl Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author E. Boye E. Boye Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. Search for more papers by this author Author Information S.J. Séror1, S. Casarégola1, F. Vannier1, N. Zouari1, M. Dahl1 and E. Boye1 1Institut de Génétique et Microbiologie, URA CNRS 1354, Université Paris XI, Orsay, France. The EMBO Journal (1994)13:2472-2480https://doi.org/10.1002/j.1460-2075.1994.tb06532.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A Bacillus subtilis mutant spnA95 was isolated as resistant at 30 degrees C to the protein kinase C (PKC) inhibitor, sphinganine, and temperature sensitive for growth. As deduced by flow cytometry measurements, the mutant has a 35% reduced initiation mass at permissive temperature, resulting in initiation of DNA replication much earlier in the cell cycle than in the wild type. This modification is accompanied by a change in cell size, as determined by phase-contrast microscopy and flow cytometry. Therefore, this strain displays the characteristics of a novel cell clock mutant. spnA is a newly identified gene in B.subtilis and was shown to encode a cysteinyl-tRNA synthetase. At non-permissive temperature, the mutant was defective in the synthesis of P70, a protein with several characteristics of PKC (a cysteine-rich protein). As one possibility, we propose that the altered timing of replication may be due to the reduced synthesis of specific cysteine-rich proteins normally involved in controlling chromosomal replication initiation in B. subtilis. Next ArticlePrevious Article Volume 13Issue 101 May 1994In this issue RelatedDetailsLoading ...