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[42] Mast cells and mast cell products

1988; Academic Press; Linguagem: Inglês

10.1016/0076-6879(88)62100-8

1557-7988

Frank M. Graziano,

Biotin and Related Studies

Publisher Summary This chapter discusses the techniques utilized for isolating and purifying mast cells from tissues and animals in which the inflammatory process is being studied. Methods used for measuring some of the chemical mediators contained in mast-cell granules and shown to have importance in inflammation and many methods for assessing the results of the micropore chemotactic assay are described. These fall into three basic types: (1) counting the number of cells that have arrived at a preselected distance, such as the distal surface of the filter, (2) measurement of the distance migrated by faster migrating cells (leading-front technique), and (3) measurement of the average distance migrated per cell . The first method described is very sensitive for detecting differences in nonstimulated and chemotactic responses. It can be used effectively for the measurement of chemotactic activity released from mast cells. The latter two methods are much more sensitive to defects in cell migration and are used for the investigation of clinical defects in this process.