Portal de Periódicos da CAPES

Chapter 18 Prevention of neural allograft rejection in the mouse following in vivo depletion of L3T4 + but not LYT-2 + T-lymphocytes

1990; Elsevier BV; Linguagem: Inglês

10.1016/s0079-6123(08)62601-1

1875-7855

M. Kelly Nicholas, Andrew G. Chenelle, Margaret M. Brown, Kāri Stefánsson, B. G. W. Arnason,

Reproductive System and Pregnancy

This chapter examines the prevention of neural allograft rejection in a mouse following an in vivo depletion of L3T4 + but not LYT-2 + T-lymphocytes. In the experiment described in the chapter, a mouse model for fetal neural transplantation as a means of determining the degree of immunological privilege afforded neural tissue following transplantation to the central nervous system (CNS). Although L3T4 + T-cells consistently outnumbered LYT-2 + T-cells in isolates from neural allograft, the relative numbers of LYT-2 + cells increased with time such that the L3T4:LYT-2 ratio decreased from 5.2 at 1 month after transplantation to 2.2 at 4 months after transplantation. This suggested a possible role for the LYT-2 T-lymphocyte late in the rejection process. The success of T-cell depletion in vivo was established using flow cytometry. The results of a blinded histological evaluation of transplants from the 4 treatment groups are presented in the chapter.