Multicompartment Cell Cultures for Studies of Neuronal Interactions
1991; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-12-185257-3.50025-2
ISSN1043-9471
AutoresElaine A. Neale, Sandra C. Fitzgerald, Linda M. Bowers, Chang Yu, R. Douglas Fields, Phillip G. Nelson,
Tópico(s)Lipid Membrane Structure and Behavior
ResumoThis chapter describes the application of multicompartment cell cultures for studies of neuronal interactions. The innate complexity of the intact nervous system inhibits a detailed mechanistic approach to the problems of synapse elimination and stabilization. Cell culture of dissociated neurons has been an extremely useful method for studying cellular mechanisms in neurons isolated from the nervous system. However, in sacrificing the complex patterns of structural connections, which link neurons into a functional system, many interesting phenomena are lost. By imposing a minimal structure on the dissociated neurons in culture, some of these interneuronal phenomena can be reproduced in a preparation that provides maximal access for experimental studies. Organ culture is one solution to this problem, but often, the accessibility and control desired for many studies is not provided by this technique. The chapter presents a description of procedures—derived from Campenot's method—that are used for constructing multicompartmented preparations for the selective stimulation, pharmacologic manipulation, and biochemical isolation of a given set of input or target neurons. The chapter also presents experimental data obtained from multicompartment cultures.
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