Portal de Periódicos da CAPES

DNA sequences from a ligninolytic filamentous fungus Phanerochaetechrysosporium capable of autonomous replication in yeast

1984; Elsevier BV; Volume: 118; Issue: 3 Linguagem: Inglês

10.1016/0006-291x(84)91468-2

1090-2104

Tekmal R. Rao, C. Adinarayana Reddy,

Fermentation and Sensory Analysis

Five different DNA sequences of Phanerochaete chrysosporium capable of supporting autonomous replication of yeast integration plasmid (YIp5) in Saccharomyces cerevisiae were isolated. These hybrid plasmids with the autonomous replication sequences from P. chrysosporium are maintained extra-chromosomally, are mitotically unstable and transform Ura3 deletion mutant of S. cerevisiae to Ura+ phenotype with high frequency. The autonomous replication sequence in pRR2, one of the recombinant plasmids, was further characterized and was shown to be homologous to P. chrysosporium genomic DNA. Restriction analyses showed that this plasmid has unique PvuII and SalI restriction sites for cloning.