Portal de Periódicos da CAPES

Molecular Evolution of the SARS Coronavirus During the Course of the SARS Epidemic in China

2004; American Association for the Advancement of Science; Volume: 303; Issue: 5664 Linguagem: Inglês

10.1126/science.1092002

1095-9203

Jian He, Peng Guo, Min Jun, De Wen Yu, Wen jia Liang, Shu Yi Zhang, Rui Heng Xu, Huan Ying Zheng, Xin Wu, Jun Xu, Zhanhui Wang, Ling Fang, Xin Zhang, Hui Li, Xin Ge Yan, Jia Lu, Zhi Hong Hu, Ji Huang, Zhuo Yue Wan, Jin Hou, Jin Lin, Huai Dong Song, Sheng Yue Wang, Xiang Zhou, Guo Wei Zhang, Bo Wei Gu, Hua Jun Zheng, Xiang Lin Zhang, Mei He, Kui Zheng, Bo Wang, Gang Fu, Xiao Ning Wang, Sai Juan Chen, Chen Zhu, Pei Hao, Hua Tang, Shuang Xi Ren, Yang Zhong, Zong Ming Guo, Qi Liu, You Gang Miao, Xiang Yin Kong, Wei He, Yi Xue Li, Chung Wu, Guoping Zhao, Rossa W. K. Chiu, S Chim, Tong Yu, Paul K.S. Chan, John S. Tam, Y. M. Dennis Lo,

Viral gastroenteritis research and epidemiology

Sixty-one SARS coronavirus genomic sequences derived from the early, middle, and late phases of the severe acute respiratory syndrome (SARS) epidemic were analyzed together with two viral sequences from palm civets. Genotypes characteristic of each phase were discovered, and the earliest genotypes were similar to the animal SARS-like coronaviruses. Major deletions were observed in the Orf8 region of the genome, both at the start and the end of the epidemic. The neutral mutation rate of the viral genome was constant but the amino acid substitution rate of the coding sequences slowed during the course of the epidemic. The spike protein showed the strongest initial responses to positive selection pressures, followed by subsequent purifying selection and eventual stabilization.