Propagation and purification of RNA plant viruses
1986; Academic Press; Linguagem: Inglês
10.1016/0076-6879(86)18109-2
ISSN1557-7988
Autores Tópico(s)Plant Pathogens and Resistance
ResumoThis chapter describes the propagation and purification of RNA plant viruses, such as tobacco mosaic virus (TMV), brome mosaic virus (BMV), and tobacco etch virus (TEV). TMV and BMV are widely used and easily purified. However, TEV is a member of the potyvirus group that includes filamentous viruses. Potyviruses are present in lower concentration than the best known plant viruses and are more difficult to purify. The minipurification procedure is applicable to many viruses, but the purity of the product is unsatisfactory for many uses. BMV is easy to purify, because it is stable in weak acids that precipitate otherwise difficult to remove plant components. BMV can be propagated in almost any barley variety. TMV is easily isolated in high yield and its infectivity is very stable. TMV can be propagated in most tobacco varieties. For devising new procedures, effects of plant host, plant age, growth temperature, and age of infection on virus accumulation should be determined. In the purification procedure pH, ionic strength, redox potential, and divalent cation status (presence or absence) are important factors to control. Impure viruses can behave anomalously, because they interact with impurities.
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