Liquid Chromatographic Determination of Glyphosate in Water-Soluble Granular Formulations: Collaborative Study
1997; Oxford University Press; Volume: 80; Issue: 3 Linguagem: Inglês
10.1093/jaoac/80.3.464
ISSN1944-7922
AutoresLynn W Morlier, David F Tomkins, Benny Køppen, B Declercq, Olaf Weller, A Gregoric, Charles Focht, Gerald D. Roberts, Donald N Harbin, R H Schreuder, H.L. Hicks, F Verbist, Teresa M. Wieland, Thomas Bjorholm, L Manso, Steven A. Cohen, Eun-Yong Lee, T Zurcher, M D Muller, David F Tomkins, Alan Noble, H D Schlesinger, B Smallage, K C Tarn, YW Kim, Tomonobu Satô, David Schultz, A. Parsons, I M Galoux, Mubashir Hussain, F M E Masollerx,
Tópico(s)Pesticide and Herbicide Environmental Studies
ResumoAbstract A liquid chromatographic (LC) method for the determination of glyphosate in various water-soluble granular formulations was tested by 28 laboratories. Samples were dissolved in mobile phase and injected directly into an LC system with a 25 cm SAX column and a mobile phase of 96% aqueous buffer solution, 0.0062M KH2PO4, and 4% methanol. Detection was by UV absorption at 195 nm. Manual or automated injections were made via fixed-volume loops. Calculations were based on peak area comparisons with external standards. The collaborative study analyzed 5 matched pairs of 4 water-soluble granular formulations, with one determination per sample. The study generated 138 matched pairs, which were analyzed by using the AOAC spreadsheet and Youden’s matched-pair calculations. Coefficients of variation for the 4 formulations ranged from 0.65 to 1.37%. The LC method for the determination of glyphosate in water-soluble granular formulations has been adopted first action by AOAC INTERNATIONAL.
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