[56] Cell synchronization
1974; Academic Press; Linguagem: Inglês
10.1016/0076-6879(74)32059-9
ISSN1557-7988
Autores Tópico(s)3D Printing in Biomedical Research
ResumoThe most effective method available for obtaining cells, most of which are in mitosis, is based upon two long-known facts of cell monolayer behavior: (1) the tenacity of cell attachment to substrate is Ca2+ dependent, and (2) the typical mammalian cell rounds up when it enters mitosis relinquishing much of its substrate attachment. By maintaining monolayer cultures in medium containing no Ca2+ other than that normally present in the serum supplement, those entering mitosis become so tenuously attached that relatively gentle shearing forces are sufficient to detach them preferentially.
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