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The ovo gene of Drosophila encodes a zinc finger protein required for female germ line development.

1991; Springer Nature; Volume: 10; Issue: 8 Linguagem: Inglês

10.1002/j.1460-2075.1991.tb07762.x

1460-2075

Maryvonne Mével-Ninio, Régine Terracol, Fotis C. Kafatos,

Animal Genetics and Reproduction

Research Article1 August 1991free access The ovo gene of Drosophila encodes a zinc finger protein required for female germ line development. M. Mével-Ninio M. Mével-Ninio Centre de Génétique Moléculaire du CNRS, Gif sur Yvette, France. Search for more papers by this author R. Terracol R. Terracol Centre de Génétique Moléculaire du CNRS, Gif sur Yvette, France. Search for more papers by this author F.C. Kafatos F.C. Kafatos Centre de Génétique Moléculaire du CNRS, Gif sur Yvette, France. Search for more papers by this author M. Mével-Ninio M. Mével-Ninio Centre de Génétique Moléculaire du CNRS, Gif sur Yvette, France. Search for more papers by this author R. Terracol R. Terracol Centre de Génétique Moléculaire du CNRS, Gif sur Yvette, France. Search for more papers by this author F.C. Kafatos F.C. Kafatos Centre de Génétique Moléculaire du CNRS, Gif sur Yvette, France. Search for more papers by this author Author Information M. Mével-Ninio1, R. Terracol1 and F.C. Kafatos1 1Centre de Génétique Moléculaire du CNRS, Gif sur Yvette, France. The EMBO Journal (1991)10:2259-2266https://doi.org/10.1002/j.1460-2075.1991.tb07762.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info As defined by dominant and recessive ovo mutations, the ovo gene is required for development of the Drosophila female germ line, and does not exert any function in males or in somatic tissues. However, reversion of dominant ovo mutations can result in new phenotypes that are not related to the female germ line: the svb and lzl mutations affect cuticle and eye development, respectively. We have identified a 7.2 kb genomic fragment that rescues ovo mutations in transgenic Drosophila and thus contains all sequences necessary for ovo+ function. This fragment has been sequenced almost in its entirety, defining the ovo locus at the molecular level. Multiple copies of the same fragment also rescue the lzl mutation. They do not rescue svb mutations, in agreement with genetic evidence that the svb function requires additional, more distal sequences. Nevertheless, a number of transposable element insertions that induce a svb phenotype interrupt the coding sequence of ovo. Taken together, the genetic and molecular data indicate the existence of a complex locus, where the ovo and svb functions depend on overlapping coding sequences but distinct regulatory elements. The data also suggest a model for the lzl phenotype. Expression of ovo at the RNA level is detectable at stage 8 of oogenesis in nurse cells and persists through the rest of oogenesis and in early embryogenesis. The ovo transcript encodes a protein of at least 1209 amino acids with four zinc fingers, suggesting that ovo might be a transcription factor required for female germ line maintenance and gametogenesis. Previous ArticleNext Article Volume 10Issue 81 August 1991In this issue RelatedDetailsLoading ...